Table 4.

Effects of TT supplementation on serum amino acid metabolites in mice fed HFD compared to the HFD control group.

Sub Pathway	Biochemical Name	HFD+TT800/HFD
Glycine, Serine, and Threonine Metabolism	N-acetylglycine	0.75
	Serine	1.13
Alanine and Aspartate Metabolism	Alanine	1.17
Glutamate Metabolism	Pyroglutamine	1.15
Histidine Metabolism	Histidine	1.06
	N-acetyl-3-methylhistidine	0.68
	Imidazole propionate	0.35
	1-Methylhistamine	1.28
Lysine Metabolism	N2-acetyllysine	0.76
	N2,N6-diacetyllysine	0.83
	N6,N6,N6-trimethyllysine	0.76
	2-Aminoadipate	0.84
Phenylalanine Metabolism	Phenylpyruvate	1.28
Tyrosine Metabolism	Tyrosine	1.12
	2-Hydroxyphenylacetate	1.29
Tryptophan Metabolism	Kynurenate	0.70
	Xanthurenate	0.43
	Serotonin	1.39
	Indoleacetate	1.31
Leucine, Isoleucine, and Valine Metabolism	3-Methylglutaconate	2.00
	Alpha-hydroxyisovalerate	0.88
	Ethylmalonate	0.84
	3-Methyl-2-oxobutyrate	1.35
Methionine, Cysteine, SAM, and Taurine Metabolism	S-methylcysteine	1.42
	N-acetyltaurine	0.77
Urea cycle, Arginine, and Proline Metabolism	2-Oxoarginine	0.81
	N-monomethylarginine	1.22
Creatine Metabolism	Creatine phosphate	1.86
Guanidino and Acetamido Metabolism	4-Guanidinobutanoate	0.80
Glutathione Metabolism	2-Hydroxybutyrate/2-Hydroxyisobutyrate	0.75

Metabolite values are expressed as the ratio of HFD+TT800/HFD which is the fold change of the treated HFD+TT800 group compared to the HFD control group. A ratio greater than 1 indicates a value larger for the treated group (HFD+TT800) and less than 1 the value for HFD+TT800 group is lower compared to the HFD control group. Green indicates fold reduction and red is for fold increase by TT supplementation with p ≤ 0.05 that was obtained from the Student’s t-test. Yellow indicates fold reduction and pink is for fold increase by TT supplementation with 0.05 < p< 0.1 that was obtained from the Student’s t-test.