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. 2021 Apr 10;13(8):1815. doi: 10.3390/cancers13081815

Figure 3.

Figure 3

Five primary cell cultures proliferate when grown on Glc and Gal hexose mixtures, altering the mitochondria (A, incubation time indicated, n = 8; blue bars indicate cell count and red bars indicate Mitotracker intensity), with representative images of the Mitotracker labeling of GBM175 cells (B, scale bar 10 µm; red: Mitotracker staining, blue: DAPI staining). Representative images of the ab15311 (anti-Glut3) and/or HPA006539 (anti-Glut14) antibody labeling levels in GBM175 cells (C, scale bar 10 µm; red: Glut3 or Glut14 staining, blue: DAPI) and levels of transporters of five primary cell cultures when grown on Glc and Gal hexose mixtures (D, n = 6; blue bars: Glut3 levels, blue bars: Glut14 levels). Five primary cell cultures grown on Glc and Gal hexose mixtures for 24 h in the presence of a 1/150 dilution of ab15311 (anti-Glut3) and/or HPA006539 (anti-Glut14 (E, n = 6; green bars: Glc treatment, orange bars: Gal treatment). Five primary cell cultures were incubated for 24 h in 10 µM CDP36, a GALK1-specific inhibitor, and underwent a loss in viability only in Gal-containing, and not Glc-containing, media (F, n = 6; green bars: Glc treatment, orange bars: Gal treatment), * p < 0.05, ** p < 0.001, *** p < 0.005 and **** p < 0.0001.