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. 2021 Apr 10;10(4):864. doi: 10.3390/cells10040864

Figure 2.

Figure 2

HAP stem cells differentiated to dopaminergic neurons. The left panels show HAP stem cells cultured in non-induction medium. The right panels show HAP stem cells cultured in neural-induction medium. (A) Immunofluorescence staining shows that HAP stem cells cultured in neural-induction medium differentiated to βIII-tubulin-positive (red fluorescence) and tyrosine-hydroxylase-positive (green fluorescence) neurons. (blue fluorescence = DAPI). Tyrosine-hydrox-ylase was expressed much more strongly in neural-induction medium. (B) HAP stem cells cultured in neural-induction medium differentiated to dopamine-positive (red fluorescence) dopaminergic neurons. Dopamine was produced much more in neural-induction medium. (C) HAP stem cells cultured in neural-induction medium differentiated to DAT positive (red fluorescence). (D) HAP stem cells cultured in neural-induction medium differentiated to Nurr1 positive (red fluorescence). Scale bar = 100 μm.