Table 3.
Commercially available, chemically defined/serum-free/xeno-free media tested for expansion of hMSCs; including selected studies from 2010–2021. Information is given on brand name, supplier, any added supplements, the cell source, cultivation parameters, and stem cell characteristics.
| Basal Medium | Supplier | Add. Supplements | Cells Expanded | Cultivation Time | Outperformed CTL Medium? | Diff Capacity | Cell Surface Markers | Ref. |
|---|---|---|---|---|---|---|---|---|
| MSCGM-CD | Lonza | - | UC-MSC | 5–7 passages | n.t. | unaltered | unaltered | [38] |
| MesenCult | Stemcell Technologies | - | hESC-derived MSCs | - | yes | n.t. | unaltered | [155] |
| Mesencult-XF™ | Stemcell Technologies | - | AD-MSC, BM-MSC | - | yes | improved (AD-MSC), decreased (BM-MSC) | altered (BM-MSC) | [156] |
| StemPro MSC SFM XenoFree™ | Life Technologies | - | BM-MSC | up to p4 | no | n.t. | n.t. | [139] |
| Mesencult-XF™ | Stemcell Technologies | - | BM-MSC | up to p4 | no | unaltered | unaltered | [139] |
| BD Mosaic™ Mesenchymal Stem Cell Serum-Free media | BD Biosciences | - | BM-MSC | up to p4 | no | unaltered | unaltered | [139] |
| StemPro® MSC SFM XenoFree, Invitrogen | Life Technologies | - | AD-MSC, BM-MSC | - | yes | altered | unaltered | [157] |
| StemPro MSC SFM XenoFree™ | Life Technologies | PDGF-BB, bFGF, TGF-β1 | ASC line, BM-MSC | up to p9 | yes | unaltered | unaltered | [158] |
| StemPro® MSC SFM | Life Technologies | PDGF-BB, bFGF, TGF-β1 | BM-MSC | 8 passages | no | unaltered | unaltered | [159] |
| StemPro MSC SFM Xenofree | Life Technologies | - | BM-MSC, UC-MSC, AD-MSC | 7 days | yes | n.t. | n.t. | [160] |
| MSC Nutristem XF | Biological Industries | - | BM-MSC, UC-MSC, AD-MSC | 7 days | yes | n.t. | n.t. | [160] |
| MesenCult-XF | Stemcell Technologies | - | BM-MSC, UC-MSC, AD-MSC | 7 days | yes | n.t. | n.t. | [160] |
| StemXVivo SFM Human MSC Expansion Medium | R&D Systems | - | BM-MSC, UC-MSC, AD-MSC | 7 days | yes | n.t. | n.t. | [160] |
| RoosterNourish-MSC XF | RoosterBio, Inc. | BM-MSC | up to p5 | no | unaltered | unaltered | [161] | |
| StemMACS-MSC Expansion Media Kit XF |
Miltenyi Biotec | BM-MSC | up to p5 | no | unaltered | unaltered | [161] | |
| MSC NutriStem XF | Biological Industries | BM-MSC | up to p5 | no | unaltered | unaltered | [161] | |
| StemXVivo SFM Human MSC Expansion Medium | R&D Systems | BM-MSC | up to p5 | no | unaltered | unaltered | [161] |
(Abbreviations: AD—adipose tissue derived, Add.—additional, BM—bone marrow derived, CD—chemically defined, CTL—control, diff—differentiation, hPL - human platelet lysate, MSC—mesenchymal stem cell, n.t.—not tested, p—passage, SFM—serum free medium, UC—umbilical cord derived, XF—xeno-free).