Table 4.

Composition of three disclosed chemically defined cell culture media for MSCs.

Ref.	[144]	[145]	[150]
Basal Media	IMDM	IMDM	17.7 g/L IDMD
Supplemented With	17.91 ng bovine FGF/mL	5 mg/mL human serum albumin	5 mM l-glutamine
	2.80 mg/mL human albumin	100 μg/mL human Ex-Cyte lipoprotein	3.024 g/L sodium bicarbonate
	27. 65 µM hydrocortisone	2 μg/mL saturated human transferrin	10 mg/L rh insulin
	1.18% SITE (S4920; containing 0.5 µg/mL sodium selenite, 1.0 mg/mL bovine insulin, 0.55 mg/mL human transferrin, 0.2 mg/mL ethanolamine; 100-fold concentrate)	10 μg/mL rh insulin	10 mg/L rh transferrin
		1.0% 100 × MEM vitamins	4 g/L rh serum albumin
		0.89% MEM essential amino acids	55 μM β-mercaptoethanol
		0.4% MEM nonessential amino acids	0.1% chemically defined lipid concentrate
		1 mM sodium pyruvate	2% MEM essential amino acids solution
		1 mM GlutaMAX-I supplement	1% MEM non-essential amino acid solution
		10 μg/mL folic acid	1% Vitamins solution
		10 μM ascorbic acid 2-phosphate	0.1% trace elements solution
		1.0 μg/mL Biotin	50 μg/L hydrocortisone
		1.36 μg/mL vitamin B12 mix	50 mg/L l-ascorbic acid-2-phosphate
		500fold diluted trace element mix	5 mg/L rh fibronectin
		4 × 10−8 M FeSO4	5 μg/L progesterone
		10 μg/mL nucleoside mix (ribonucleosides, 2′-deoxyribo-nucleosides, uridine, and thymidine)	10 mg/L putrescine
			2 mg/L serotonin
			10 ng/mL rh EGF
		1.0% antibiotic/antimycotic	10 ng/mL rh basic FGF
		10–20 ng/mL rh PDGF ββ homodimer or 10−5 to 10−6 M 5-hydroxytryptamine	10 ng/mL rh PDGF
			10 ng/mL rh IGF

(Abbreviations: EGF—epidermal growth factor, FGF—fibroblast growth factor, rh—recombinant human, IGF—insulin-like growth factor, IMDM—Iscove’s modified Dulbecco’s medium, MEM—minimum essential media, PDGF—platelet-derived growth factor).