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. 2021 Apr 12;22(8):3979. doi: 10.3390/ijms22083979

Figure 3.

Figure 3

Non-uniform distribution of SBP-tagged LeishIF4E-5 in the cytoplasm and in foci surrounding the nucleus. Transgenic L. amazonensis promastigotes expressing SBP-tagged LeishIF4E-5 were fixed, permeabilized and processed for confocal microscopy. LeishIF4E-5-SBP was detected using a monoclonal antibody against the SBP tag and a secondary DyLight-labeled antibody (488 nm; green). Nuclear and kinetoplast DNA was stained using DAPI (blue). A bright field (BF) picture of the cells is on the right. The confocal analysis was repeated three times. A broad view of the field is given in Figure S4.