Table 3.
Primers used in quantitative real-time PCR (RT-qPCR).
| Target | Sequences (5’-3’) | Product Size | Annealing Temperature (˚C) | References | Note |
|---|---|---|---|---|---|
| Dendritic cells | F:AACAGCACACGCTCACTCAC R:CGATCATGTGAGCCTTGAGA |
153 | 60 | Zoccola et al. [50] | Initiate an adaptive immune response |
| C3 | F:GCAATCCTCCACAACTACAG R:ACTCTGACCTCCTGACGATAC |
93 | 59 | Mohd-Shaharuddin et al. [51] | Innate defense against common pathogens |
| CCL4 | F:TCCTCGTCTCACTCTGTCTGT R:GACCTGCCACTGTCTTCAGC |
301 | 60 | Chin et al. [36] | Chemokine attracts innate immune cells |
| MHC I | F:GGCTGTTTTTGCCGCTCTG R:GTGGACAGGTCTGGATAAAG |
112 | 60 | Chin et al. [36] | Molecule-presenting antigen for CD8+ |
| β-actin (control) |
F:TACCACCGGTATCGTCATGGA R:CCACGCTCTGTCAGGATCTTC |
126 | 60 | Chin et al. [36] | Reference gene |