Table 6.
Details of experimental design, vaccination scheme, challenge doses, and fish survival.
| Group | Number of Fish | Primary Vaccination (Day 0–5, Oral, 5% Fish Body Weight) | Booster Dose (Day 14–18, Oral, 5% Fish Body Weight | Challenge Group (10 Fish/Tank in Duplicates) | Challenge Dose/Fish (Day 28, IP, 0.1 mL/Fish) | RPS (%) |
|---|---|---|---|---|---|---|
| Control | 80 | PBS + POA | PBS + POA | Control (+PBS) | PBS | - |
| Control (+Vh) | 107 CFU Vh | - | ||||
| Control (+Sa) | 107 CFU Sa | - | ||||
| Control (+Ah) | 107 CFU Ah | - | ||||
| Vaccinated | 80 | 106 cells/kg of feed + POA | 106 cells/kg of feed + POA | Vaccinated (+PBS) | PBS | - |
| Vaccinated (+Vh) | 107 CFU Vh | 75 ± 7.07 | ||||
| Vaccinated (+Sa) | 107 CFU Sa | 80 ± 0 | ||||
| Vaccinated (+Ah) | 107 CFU Ah | 80 ± 0 |
Vh, Vibrio harveyi; Sa, agalactiae; Ah, Aeromonas hydrophila; POA, palm oil adjuvant; RPS, relative percent survival; -, not applicable.