Table 2.
qPCR-determined log10 cpn60 gene copies in wheat seed wash samples spiked with varying amounts of cpn60 plasmids from non-endogenous microorganisms. Two microorganisms previously shown to be associated with the wheat seed microbiota, along with the total number of 16S gene copies (representing non-cpn60 DNA) are also shown. Results are shown prior to hybridization (pre-hyb) and after hybridization (post-hyb). The observed ratio of cpn60/16S genes is shown in brackets for each target.
| Spike Level | High | Medium | Low | Unspiked | ||||
|---|---|---|---|---|---|---|---|---|
| Microorganism | Pre-Hyb | Post-Hyb | Pre-Hyb | Post-Hyb | Pre-Hyb | Post-Hyb | Pre-Hyb | Post-Hyb |
| G. vaginalis 1 | 7.18 (0.85) | 7.58 (1.36) | 6.25 (0.72) | 6.87 (1.17) | 5.22 (0.61) | 5.54 (0.99) | 0.00 (0.00) | 0.00 (0.00) |
| L. crispatus 1 | 7.20 (0.85) | 7.49 (1.35) | 6.26 (0.72) | 6.81 (1.16) | 5.28 (0.61) | 5.45 (0.98) | 0.00 (0.00) | 0.00 (0.00) |
| L. gasseri 1 | 7.36 (0.87) | 8.29 (1.49) | 6.34 (0.73) | 7.56 (1.28) | 5.37 (0.62) | 6.33 (1.14) | 0.00 (0.00) | 0.00 (0.00) |
| A. vaginae 1 | 7.24 (0.86) | 7.46 (1.34) | 6.36 (0.73) | 6.77 (1.15) | 5.39 (0.63) | 5.49 (0.99) | 0.00 (0.00) | 0.00 (0.00) |
| L. iners 1 | 7.11 (0.84) | 7.42 (1.33) | 6.21 (0.72) | 6.59 (1.12) | 5.28 (0.61) | 5.45 (0.98) | 0.00 (0.00) | 0.00 (0.00) |
| P. agglomerans 2 | 4.98 (0.59) | 6.43 (1.16) | 5.09 (0.59) | 6.78 (1.15) | 5.04 (0.58) | 6.44 (1.16) | 5.20 (0.61) | 6.51 (1.13) |
| Alternaria sp. 2 | 5.36 (0.63) | 4.76 (0.86) | 5.55 (0.64) | 5.19 (0.88) | 5.55 (0.64) | 4.96 (0.89) | 5.61 (0.65) | 5.12 (0.89) |
| 16S 2 | 8.46 | 5.56 | 8.67 | 5.89 | 8.62 | 5.56 | 8.59 | 5.74 |
1 exogenous targets (vaginal bacteria cpn60 plasmids). 2 endogenous targets (seed wash microorganisms).