Table 3.
Correlations between cpn60 gene copies determined by species-specific quantitative PCR and the number of sequencing reads mapping to each taxonomic cluster by CaptureSeq or amplification for 5 bacteria from a synthetic community spiked into a background of wheat seed washes. Samples were spiked at 4 levels (high, medium, low, unspiked) using cpn60 plasmids from bacteria exogenous to the seed washes and quantified using qPCR and by read numbers determined from CaptureSeq and marker amplification. Significant correlations (p < 0.01) are shown in bold.
| Pearson (r2) | |||
|---|---|---|---|
| Organism | CaptureSeq | Amplification | n |
| Gardnerella vaginalis | 0.999 | 0.724 | 4 |
| Lactobacillus iners | 0.998 | 0.926 | 4 |
| Lactobacillus crispatus | 0.998 | 0.922 | 4 |
| Lactobacillus gasseri | 1.000 | 0.937 | 4 |
| Atopobium vaginae | 1.000 | 0.782 | 4 |
| Spearman (ρ) | |||
| All combined | 0.956 | 0.912 | 20 |