Table 3.
Sequences of the oligonucleotide primers used in the current study and their details.
| Primer Name | Sequence (5’→3’) | Description |
|---|---|---|
| Sc-frr-F | ATAGCCATATGATGGAGAAGGCCGTCGTGGTC | Primers for the amplification of frr from S. clavuligerus to prepare pSET-frr |
| Sc-frr-R | CTTACGGATCCTCAGACTTCGAGCAGCTCGG | |
| Sc-rpsL-F | ATAGCCATATGGTGCCTACGATCCAGCAGC | Primers for the amplification of rpsL from S. clavuligerus to prepare pSET-rpsL |
| Sc-rpsL-R | CTTACGGATCCTTACTTCTCCTTCTTGGCG | |
| Sc-rpsL-K88E-F | CCGGCAGGTCCTCCACACGGCCACC | Primers for introducing a single amino acid mutation (Lys88Glu) in rpsL from S. clavuligerus to prepare pSET-rpsL-K88E |
| Sc-rpsL-K88E-R | GGTGGCCGTGTGGAGGACCTGCCGG | |
| Sc-rpsL-L90K-F | TAACGAACACCCGGCTTGTCCTTCACACGGCC | Primers for introducing a single amino acid mutation (Leu90Lys) in rpsL from S. clavuligerus to prepare pSET-rpsL-L90K |
| Sc-rpsL-L90K-R | GGCCGTGTGAAGGACAAGCCGGGTGTTCGTTA | |
| Sc-rpsL-R94G-F | CGGATGATCTTGTAACCAACACCCGGCAGGTC | Primers for introducing a single amino acid mutation (Arg94Gly) in rpsL from S. clavuligerus to prepare pSET-rpsL-R94G |
| Sc-rpsL-R94G-R | GACCTGCCGGGTGTTGGTTACAAGATCATCCG |