Figure 4.

Dual MEKi plus ERKi prevents the emergence of drug resistance. (A). Examples of colony forming assays of the indicated cell lines cultivated for 14 days with the indicated inhibitors (1 μM). (B). Long-term drug resistance assay. The indicated sub-confluent cell lines (5 × 10⁵ cells/6 cm plate) were cultivated with the indicated inhibitors (1 μM). Days to resistance represents the timepoint at which cells were first split and continued proliferation thereafter, cells that did not survive the treatments or proliferate within 4 months are depicted in blue.