Figure 6.

Cells resistant to MEKi or ERKi are frequently resistant to double inhibition. (A). Depiction of workflow used to generate secondary resistant cell lines. (B). Long-term drug resistance assay. The indicated sub-confluent cell lines (5 × 10⁵ cells/6 cm plate) were cultivated with the indicated inhibitors (1 μM). Days to resistance represents the timepoint at which cells were first split and continued proliferation thereafter, cells that did not survive the treatments or proliferate within 4 months are depicted in blue.