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. 2021 Apr 13;22(8):4002. doi: 10.3390/ijms22084002

Figure 4.

Figure 4

Effects of kurarinone on frequency of Th1 (CD4+ IFN-γ+ T cells), Th17 cells (CD4+ IL-17+ T cells) and Treg (CD4+Foxp3+ T cells) in the inguinal lymph nodes (ILNs). (A) Single cell suspensions were collected from ILNs on day 42, followed by stimulation with anti-CD3+ for 96 h and then stained with anti-CD4, anti-IFN-γ, anti-IL-17A, or anti-Foxp3 Abs and analyzed by flow cytometry. Statistical analysis of the percentages of Th1, Th1, and Treg in the LN. (B) The mRNA relative expressions of specific transcription factors for different CD4+ T cells subsets were calculated by quantitative RT-PCR using the ΔΔ CT method with the normal control group as calibrator. Data are presented as mean ± SEM of 6 mice from one of three experiments. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001 versus vehicle-treated CIA mice group (One Way ANOVA followed by Tukey’s multiple comparison test).