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. 2021 Apr 11;22(8):3934. doi: 10.3390/ijms22083934

Figure 3.

Figure 3

4-Phenylbutyrate and Inositol-Requiring Enzyme 1 (IRE1) silencing alleviated gefitinib-induced apoptosis in H4 cells. H4 cells were treated with gefitinib (0 and 40 μM) together, with or without 4-phenylbutyrate (500 μM, A–C). Cell viability (24 h) was measured with the alamarBlue assay (A). Caspase 3 activity (6 h) was measured with enzymatic assay (B). Protein extracts (6 h) were subjected to Western blot with indicated antibodies. Representative blots of three independent experiments and quantitative data are shown (C). H4 cells were transfected with control siRNA (1 nM) or IRE1 siRNA (1 nM) for 24 h. Protein extracts were subjected to Western blot with indicated antibodies. Representative blots of three independent experiments and quantitative data are shown (D). The transfected cells were then treated with gefitinib (0 and 40 μM). Cell viability (24 h) was measured with the alamarBlue assay (E). Caspase 3 activity (6 h) was measured with enzymatic assay (F). Protein extracts (6 h) were subjected to Western blot with indicated antibodies. Representative blots of three independent experiments and quantitative data are shown (G). Protein contents were normalized with corresponding total protein or GAPDH. * p < 0.05 vs. untreated control and # p < 0.05 vs. gefitinib (40 μM), n = 4 (A,B,E,F).