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. 2021 Apr 11;22(8):3934. doi: 10.3390/ijms22083934

Figure 6.

Figure 6

Noxa is crucial to gefitinib-induced apoptosis in glioma cells. H4 (A) and U87 (E) cells were treated with gefitinib (0 and 40 μM) together, with or without SP600125 (10 μM). Protein extracts (6 h) were subjected to Western blot with indicated antibodies. Representative blots of three independent experiments and quantitative data are shown. H4 (B–D) and U87 (F–H) cells were transfected with control siRNA (1 nM) or Noxa siRNA (1 nM) for 24 h. Protein extracts were subjected to Western blot with indicated antibodies. Representative blots of three independent experiments and quantitative data are shown (B,F). The transfected cells were then treated with gefitinib (0 and 40 μM). Cell viability (24 h) was measured with the alamarBlue assay (C,G). Caspase 3 activity (6 h) was measured with enzymatic assay (D,H). Protein contents were normalized with GAPDH. * p < 0.05 vs. untreated control and # p < 0.05 vs. gefitinib (40 μM), n = 4 (C,D,G,H).