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. 2021 Apr 9;13(8):1799. doi: 10.3390/cancers13081799

Figure 3.

Figure 3

MSI1 expression is highly correlated with MIF1 in GBM tumor mouse models. (A) Immunohistostaining of MSI1 and MIF1 in cross-sections of intracranial tumors derived from xenografts of the DBTRG-05MG cells transfected with Flag–MSI1 and the control Flag-only construct. H&E staining (left panels) shows general tumor morphology. (B) Quantification of MSI1—and MIF1—stained areas in (A). Mean values are shown, n = 3, SD—error bars, * p < 0.05 (Student’s t-test). (C) Western blot showing the knockout of MSI1 expression in the GL261-Luc cells transfected with Cas9 only (control) or with CRISPR/Cas9 (KO MSI1). GAPDH—loading control. (D) Control and KO MSI1 GL261-Luc cells were subjected to an intracranial injection in 8-week-old male C57BL/6 mice (n = 3). Bioluminescent signal visualization (IVIS) of tumors derived from control and KO MSI1 GL261-Luc cells in mice on day 14 post-injection is show€(E) Quantification of the bioluminescence signal from tumors in (D); n = 3, SD—error bars, * p < 0.05 (Student’s t-test).