Figure 3.
Uptake of albumin-loaded EVs in Raw 264.7 macrophages and MDA-MB-231 breast cancer cells. MDA-MB-231 human breast cancer cell-derived EVs were loaded with PULSin/albumin (albumin was labeled with Alexa Fluor 488). SEC was used to remove free albumin, PULSin and PULSin/albumin. The albumin amount in each sample loaded through SEC was equivalent and cells were treated with an equal volume of the elution fraction 7–9. Cellular uptake was assessed by flow cytometry after a three-hour incubation period. a Uptake of albumin-loaded EVs and controls in Raw 2647 cells. b Uptake of albumin-loaded EVs (103 EVs/cell) and controls in MDA-MB-231 cells. Data are presented as mean ± SD of three replicates. Statistical analysis was performed by two-way (a) or one-way ANOVA (b) with post-hoc pairwise comparisons using Tukey’s test. ****, p < 0.0001 in comparison to PULSin/albumin SEC; ####, p < 0.0001 in comparison to albumin (SEC) or PULSin/albumin (SEC).