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. 2021 Apr 10;22(8):3925. doi: 10.3390/ijms22083925

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Amino acid sequence alignment of mouse Eya1 with the predicted proteins from two choanoflagellate species that contain a putative EYA domain. Characteristic HAD sequence motifs are shown above the alignment in green letters. The ED is indicated by the green line. BLAST searches were performed using www.ncbi.nlm.nih.gov/blast (accessed on 20 February 2021). domain enhanced lookup time accelerated BLAST(DELTA-BLAST) was used to find ED domains. Alignments were obtained using MULTALIGN (www.multalign.tolouse.fr) (accessed on 20 February 2021) and CLUSTAL Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) (accessed on 20 February 2021).