Table 2.
List of the variable importance in the projection (VIPs) identify by OPLS-DA analysis performed on TuMV-inoculated resistant and susceptible twenty-six accessions in 2015. For each VIP, comparisons between resistant (R) and susceptible (S) accessions were done. The fold change was calculated for each VIP. Primary metabolites are light-grey highlighted. Metabolites that accumulate significantly more in resistant accessions are at the bottom of the table.
| VIP OPLS-DA 1 | VIP Values | m/z2 | rt 3 | Resistant vs. Susceptible Metabolic Contents | Fold Change |
|---|---|---|---|---|---|
| Sucrose | 2.213 | NA | NA | S > R *** 4 | 2.89 |
| 303;463 | 1.973 | 303.133 | 463.27 | S > R *** | 3.69 |
| 356;452 | 1.958 | 356.12 | 452.352 | S > R *** | 2.17 |
| 219;311 | 1.94 | 219.101 | 311.134 | S > R *** | 4.81 |
| 533;392 | 1.926 | 533.155 | 391.765 | S > R *** | 4.98 |
| 332;430 | 1.898 | 332.132 | 430.481 | S > R *** | 3.78 |
| 116;100 | 1.867 | 116.07 | 100.163 | S > R *** | 6.05 |
| 205;242 | 1.837 | 205.097 | 242.121 | S > R *** | 2.51 |
| 103;242 | 1.799 | 103.041 | 242.089 | S > R *** | 2.86 |
| 302;506 | 1.796 | 302.102 | 506.314 | S > R *** | 5.76 |
| 255;544 | 1.769 | 255.112 | 543.799 | S > R *** | 9.96 |
| 221;216 | 1.748 | 221.092 | 215.744 | S > R *** | 3.13 |
| 385;210 | 1.738 | 385.106 | 210.083 | S > R *** | 45.77 |
| Glucose | 1.734 | NA | NA | S > R *** | 2.49 |
| 175;402 | 1.713 | 175.148 | 401.831 | S > R *** | 3.77 |
| 903;319 | 1.71 | 903.277 | 318.889 | S > R *** | 8.48 |
| 503;391 | 1.695 | 503.19 | 391.457 | S > R *** | 2.51 |
| 343;345 | 1.693 | 343.117 | 345.319 | S > R *** | 2.08 |
| Fructose | 1.683 | NA | NA | S > R *** | 2.49 |
| 474;107 | 1.675 | 474.218 | 107.298 | S > R *** | 2.68 |
| 315;448 | 1.674 | 315.133 | 447.821 | S > R *** | 2.39 |
| 209;488 | 1.669 | 209.153 | 488.35 | S > R *** | 2.7 |
| 209;414 | 1.663 | 209.153 | 413.756 | S > R *** | 2.14 |
| 212;284 | 1.657 | 211.559 | 283.891 | S > R *** | 1.56 |
| 543;99 | 1.645 | 543.132 | 98.5221 | S > R *** | 5.84 |
| 124;346 | 1.638 | 124.075 | 346.354 | S > R *** | 1.92 |
| 370;302 | 1.61 | 370.149 | 301.519 | S > R *** | 28.49 |
| 203;204 | 1.591 | 203.084 | 203.918 | S > R *** | 8.24 |
| Glutamate | 1.59 | NA | NA | S > R *** | 1.47 |
| 430;333 | 1.581 | 430.17 | 332.878 | S > R *** | 3.3 |
| 331;329 | 1.578 | 331.117 | 328.817 | S > R *** | 1.58 |
| 226;406 | 1.554 | 226.107 | 406.369 | S > R *** | 7.97 |
| 151;359 | 1.549 | 151.075 | 359.377 | S > R *** | 1.78 |
| 449;319 | 1.545 | 449.106 | 318.949 | S > R *** | 1.75 |
| 757;319 | 1.537 | 757.217 | 319.13 | S > R *** | 1.69 |
| 270;587 | 1.534 | 270.133 | 587.091 | S > R *** | 43.68 |
| 315;370 | 1.524 | 315.133 | 370.476 | S > R *** | 3.93 |
| 162;402 | 1.511 | 162.055 | 402.281 | S > R *** | 1.9 |
| 221;230 | 1.503 | 221.121 | 230.244 | S > R *** | 1.74 |
| 394;517 | 1.49 | 394.204 | 517.072 | S > R *** | 17.28 |
| 302;407 | 1.486 | 302.101 | 406.697 | S > R *** | 7.16 |
| 482;101 | 1.475 | 482.107 | 101.274 | S > R *** | 1.79 |
| Amino Acids | 1.462 | NA | NA | S > R *** | 1.88 |
| 355;306 | 1.451 | 355.102 | 306.288 | S > R *** | 3.29 |
| 191;416 | 1.448 | 191.143 | 416.467 | S > R *** | 2.49 |
| 193;324 | 1.445 | 193.125 | 323.817 | S > R *** | 1.46 |
| 149;360 | 1.437 | 149.096 | 360.18 | S > R *** | 1.66 |
| 642;445 | 1.435 | 642.254 | 445.078 | S > R *** | 2 |
| 321;760 | 1.419 | 321.114 | 760.442 | S > R *** | 3.7 |
| 182;466 | 1.415 | 182.081 | 465.796 | S > R *** | 4.21 |
| 305;210 | 1.408 | 305.086 | 210.166 | S > R *** | 2.24 |
| 348;319 | 1.396 | 348.274 | 318.945 | S > R *** | 3.61 |
| 164;496 | 1.375 | 164.07 | 496.307 | S > R *** | 2.22 |
| 165;424 | 1.372 | 165.127 | 424.14 | S > R *** | 1.54 |
| 317;464 | 1.37 | 317.101 | 463.65 | S > R *** | 1.25 |
| 191;363 | 1.36 | 191.07 | 362.643 | S > R *** | 2.76 |
| 219;486 | 1.34 | 219.101 | 486.477 | S > R *** | 2.7 |
| 105;700 | 1.33 | 105.069 | 700.287 | S > R *** | 1.59 |
| 367;358 | 1.323 | 367.101 | 358.491 | S > R *** | 1.69 |
| 201;344 | 1.319 | 201.054 | 343.832 | S > R *** | 1.71 |
| 189;325 | 1.312 | 189.127 | 324.754 | S > R *** | 1.74 |
| 146;242 | 1.311 | 146.06 | 241.843 | S > R *** | 2.31 |
| 133;296 | 1.307 | 133.064 | 295.68 | S > R *** | 2.65 |
| 179;604 | 1.306 | 179.106 | 603.961 | S > R *** | 1.64 |
| 374;322 | 1.305 | 374.144 | 321.703 | S > R *** | 1.77 |
| 373;453 | 1.29 | 373.127 | 452.6 | S > R *** | 1.81 |
| 302;382 | 1.282 | 302.041 | 381.707 | S > R *** | 1.54 |
| 161;442 | 1.274 | 161.096 | 441.797 | S > R *** | 1.79 |
| 164;375 | 1.269 | 164.07 | 375.012 | S > R *** | 2.95 |
| 109;359 | 1.267 | 109.064 | 359.34 | S > R *** | 1.92 |
| 386;278 | 1.256 | 386.22 | 277.549 | S > R *** | 3.48 |
| 391;774 | 1.255 | 391.245 | 774.477 | S > R *** | 2.29 |
| 409;491 | 1.251 | 409.169 | 490.545 | S > R *** | 27.12 |
| 291;259 | 1.241 | 291.181 | 259.063 | S > R *** | 4.78 |
| 192;462 | 1.227 | 192.041 | 462.37 | S > R *** | 1.27 |
| 107;370 | 1.219 | 107.085 | 370.001 | S > R *** | 1.39 |
| 420;276 | 1.217 | 419.695 | 275.797 | S > R *** | 1.77 |
| 193;373 | 1.189 | 193.086 | 373.266 | S > R *** | 2.04 |
| 379;402 | 1.179 | 379.095 | 402.311 | S > R *** | 2.88 |
| 181;464 | 1.176 | 181.086 | 463.916 | S > R *** | 1.42 |
| 181;328 | 1.17 | 181.086 | 327.894 | S > R *** | 1.68 |
| 80;395 | 1.168 | 80.049 | 395.289 | S > R *** | 1.57 |
| 373;344 | 1.16 | 373.127 | 343.531 | S > R *** | 1.54 |
| 627;299 | 1.156 | 627.155 | 299.369 | S > R *** | 2.8 |
| 96;389 | 1.155 | 96.08 | 389.004 | S > R *** | 1.74 |
| 195;453 | 1.154 | 195.065 | 452.614 | S > R *** | 1.55 |
| 79;396 | 1.152 | 79.041 | 395.754 | S > R *** | 1.52 |
| 105;327 | 1.15 | 105.069 | 326.898 | S > R *** | 1.72 |
| 210;465 | 1.147 | 210.112 | 464.799 | S > R *** | 2.44 |
| 335;506 | 1.143 | 335.127 | 506.441 | S > R *** | 3.71 |
| 86;126 | 1.114 | 86.096 | 126.246 | S > R *** | 2.09 |
| 396;257 | 1.106 | 396.185 | 256.51 | S > R *** | 1.43 |
| 611;354 | 1.102 | 611.158 | 353.603 | S > R *** | 1.34 |
| 178;191 | 1.102 | 178.089 | 191.013 | S > R *** | 3.43 |
| 169;496 | 1.101 | 169.049 | 495.5 | S > R *** | 3.67 |
| 162;249 | 1.098 | 162.055 | 249.101 | S > R *** | 1.5 |
| 396;348 | 1.092 | 396.115 | 347.739 | S > R *** | 4.14 |
| 103;389 | 1.086 | 103.054 | 389.444 | S > R *** | 2 |
| 521;325 | 1.084 | 521.201 | 325.328 | S > R *** | 2.52 |
| 133;126 | 1.072 | 133.105 | 126.347 | S > R ** | 2.32 |
| 209;426 | 1.071 | 209.153 | 425.939 | S > R *** | 1.98 |
| 162;357 | 1.069 | 162.055 | 356.879 | S > R *** | 1.74 |
| 201;451 | 1.063 | 201.054 | 451.123 | S > R *** | 1.75 |
| 527;460 | 1.056 | 527.103 | 460.095 | S > R *** | 2.02 |
| Fumarate | 1.054 | NA | NA | S > R *** | 1.38 |
| 393;416 | 1.052 | 393.188 | 415.816 | S > R *** | 1.71 |
| 212;774 | 1.052 | 212.094 | 774.047 | S > R *** | 2.76 |
| 402;420 | 1.048 | 402.162 | 419.597 | S > R ** | 1.87 |
| 404;388 | 1.038 | 404.227 | 388.155 | S > R ** | 1.31 |
| 464;367 | 1.035 | 464.248 | 366.989 | S > R *** | 1.97 |
| 225;371 | 1.034 | 225.148 | 370.798 | S > R *** | 1.57 |
| 103;284 | 1.033 | 103.054 | 283.823 | S > R *** | 3.26 |
| 227;789 | 1.023 | 227.163 | 788.506 | S > R *** | 1.61 |
| 222;216 | 1.016 | 221.602 | 215.822 | S > R ** | 1.33 |
| 367;342 | 1.011 | 367.153 | 341.908 | S > R *** | 1.96 |
| 302;463 | 1.006 | 302.102 | 463.078 | S > R *** | 1.49 |
| 309;325 | 1.003 | 309.116 | 324.659 | S > R ** | 1.44 |
| 244;325 | 1.001 | 244.096 | 324.677 | S > R ** | 1.41 |
| 351;184 | 2.047 | 351.006 | 184.222 | R > S *** | 2.88 |
| 432;184 | 1.831 | 431.971 | 183.776 | R > S *** | 3.44 |
| 137;131 | 1.618 | 136.931 | 131.31 | R > S *** | 1.39 |
| 512;491 | 1.563 | 512.127 | 491.367 | R > S *** | 2.54 |
| 394;108 | 1.548 | 394.2 | 108.419 | R > S *** | 1.93 |
| 337;573 | 1.51 | 337.292 | 572.917 | R > S *** | 1.64 |
| 299;181 | 1.396 | 299.098 | 181.363 | R > S *** | 22.3 |
| 181;139 | 1.293 | 181.053 | 138.934 | R > S *** | 1.94 |
| 324;184 | 1.263 | 323.989 | 183.797 | R > S *** | 106.8 |
| 280;184 | 1.229 | 280.084 | 183.768 | R > S *** | 5.9 |
| 361;491 | 1.218 | 361.092 | 490.564 | R > S *** | 2.07 |
| 155;132 | 1.141 | 154.941 | 131.541 | R > S *** | 1.33 |
| 433;482 | 1.114 | 433.112 | 481.588 | R > S *** | 244 |
| 433;700 | 1.109 | 433.241 | 700.261 | R > S *** | 1.82 |
| 64;395 | 1.091 | 63.934 | 395.047 | R > S *** | 3.6 |
| 256;668 | 1.089 | 256.08 | 667.874 | R > S *** | 2.12 |
| 460;740 | 1.076 | 460.269 | 740.005 | R > S ** | 1.42 |
| 449;442 | 1.069 | 449.107 | 442.311 | R > S *** | 6.17 |
| 244;130 | 1.049 | 243.942 | 130.167 | R > S *** | 1.35 |
| 347;240 | 1.039 | 347.159 | 239.659 | R > S ** | 1.48 |
| 86;184 | 1.015 | 86.059 | 184.278 | R > S *** | 1.87 |
1 When undetermined, VIP are identified through mz/rt values. VIP values are classified in decreasing order, 2 mass to charge ratio, 3 retention time. 4 The significance was assessed through a Wilcoxon test at *** p < 0.001, ** 0.001 < p < 0.01.