Table 1.
Research groups of experimental animals. Control groups: I, Ia, and other groups with diet modified by the addition of Ilex extracts (II–VI) or both Ilex extracts and cholesterol (IIa–VIa).
Group | Diet Type (8 Animals in Each Diet Group) |
---|---|
I | rats fed with a standard diet |
Ia | rats fed as a group I but with the addition of 20 g of cholesterol per kilogram of diet |
II | rats receiving instead of drinking water the water extract of I. paraguariensis (each day, freshly infused extract was prepared by extraction of 50 g of leaves with 1L boiled water; every two animals had free access to 250 mL of this sole source of drink per day) |
IIa | rats fed as group II but with the addition of 20 g of cholesterol per kilogram of diet |
III | rats receiving instead of drinking water the water extract of I. meserveae “Blue Angel” (each day, freshly infused extract was prepared by extraction of 50 g of leaves with 1L boiled water; every two animals had free access to 250 mL of this sole source of drink per day) |
IIIa | rats fed as group III but with the addition of 20 g of cholesterol per kilogram of diet |
IV | rats receiving additionally polyphenol fraction from I. meserveae “Blue Angel” (each day, the dry extract was freshly solubilized in water in a dose of 10 mg/kg BW; every two animals had free access to 250 mL of this sole source of drink per day) |
IVa | rats fed as group IV but with the addition of 20 g of cholesterol per kilogram of diet |
V | rats receiving additionally terpenoid fraction from I. meserveae “Blue Angel” (each day, 200 mL of oil was mixed with terpenoids (in a dose 10 mg/kg BW) and 1 kg of feed and left overnight; every two animals had free access to diet and drinking water, supplied ad libitum as in group I) |
Va | rats fed as group V but with the addition of 20 g of cholesterol per kilogram of diet |
VI | rats receiving additionally saponin fraction from I. meserveae “Blue Angel” (each day, the dry extract was freshly solubilized in water in a dose of 10 mg/kg BW; every two animals had free access to 250 mL of this sole source of drink per day) |
VIa | rats fed as group VI but with the addition of 20 g of cholesterol per kilogram of diet |