Distribution of DNA methylation pattern and biological characteristics in HPV-associated OPSCC. Genome-wide DNA methylation analysis revealed two types of DNA methylation patterns in HPV-associated OPSCC. In high DNA methylation subtype, HPV genome integration (−), episomal HPV (+), high expression of E2/E4/E5, and better prognosis compared to the intermediate DNA methylation subtype and CREBBP mutation (+) were previously reported. In intermediate DNA methylation subtype, HPV genome integration (+), episomal HPV (−), high expression of E6/E7, and worse prognosis compared to the high DNA methylation subtype were reported.