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High performance liquid chromatography (HPLC) conditions to determine phenolics.
| Time (min) | Flow Rate (mL/min) | Solvent A (%) | Solvent B (%) | Solvent C (%) |
|---|---|---|---|---|
| 0 | 0.6 | 90 | 6 | 4 |
| 5 | 0.6 | 85 | 9 | 6 |
| 30 | 0.6 | 71 | 17.4 | 11.6 |
| 60 | 0.6 | 0 | 85 | 15 |
| 61 | 0.6 | 90 | 6 | 4 |
| 66 | 0.6 | 90 | 6 | 4 |
Solvent A = Milli-Q water containing 0.05% (v/v) trifluoroacetic acid (TFA); solvent B = methanol containing 0.05% (v/v) TFA; solvent C = acetonitrile containing 0.05% (v/v) TFA.
