Table 1.
Immobilisation of DPEase on HFA.
| Treatment | Optimum Immobilisation Condition | Immobilisation Results | |||||
|---|---|---|---|---|---|---|---|
| Applied Enzyme Loads (U/g Dry Resin) | Time (h) | Temperature (°C) | pH | Enzyme Activity (U/g Dry Resin) | Activity Recovery (%) | Protein Loading Efficiency (%) | |
| Ion exchange | 200 | 8 | 20 | 7.5 | 65.6 ± 4.0 D | 32.8± 2.0 D | 58.2 ± 2.2 C |
| Ion exchange + Covalent binding | 200 | 8 + 12 | 20 | 7.5 | 89.2 ± 3.2 C | 44.6 ± 1.6 C | 77.5 ± 3.1 B |
| Ion exchange + Covalent binding + Crosslinking | 200 | 8 + 12 + 1 | 20 | 7.5 | 138.8± 2.6 A | 69.4 ± 1.3 A | 90.6 ± 2.0 A |
| Ion exchange + Covalent binding + Crosslinking + Blocking | 200 | 8 + 12 + 1 + 16 | 20 | 7.5 | 103.5 ± 2.6 B | 51.8 ± 1.3 B | 90.6 ± 2.0 A |
Data followed by different superscript letters on the same column denote statistically significant differences (p < 0.05).