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. 2021 Apr 12;10(4):876. doi: 10.3390/cells10040876

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Long-term cultured CDK8/19i-naïve hPSCs retain monoallelic expression and hemi-methylation at imprinted loci. (A) Heatmaps of allele specific expression data for the indicated informative imprinted genes in primed versus CDK8/19i long-term cultured hPSCs. The lower part of each panel indicates the number and percentage of genes expressed in a monoallelic or partially monoallelic manner after long-term culture in the presence of CDK8/19i. (B) CpG methylation status of differentially methylated regions (DMRs) at the indicated imprinted loci in five hPSC lines adapted to CDK8/19i for >10 passages. For each DMR, a total of 4 or 5 individual CpGs were analyzed by pyrosequencing. (C) CpG methylation status of DMRs in teratomas obtained from primed hPSCs or long-term adapted to CDK8/19i.