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Anchorage-independent growth. HeLa cells and E1-MYC cells from pre- or post-production medium were seeded at three different densities and cultured for 7 days. (a) Representative images (4× magnification) HeLa and E1-MYC cultures after 7 days. (b) Relative cell growth as determined by DNA-binding dye fluorescence after 7 days in culture. Results represent the mean (n = 3, triplicate for each sample) ± SD.
