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. 2021 Apr 12;22(8):3954. doi: 10.3390/ijms22083954

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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PD measurement and tear fluid volume measurement of isorhamnetin in live mice. (A,B) Representative continuous recordings of change in ocular surface open-circuit PD in response to perfusion with solutions containing indicated compounds (see Section 4). (C) Summary of changes in ocular surface PD (ΔPD). (D) Tear volume was measured at the indicated times after 2.5 μL volume of single ocular delivery of vehicle, isorhamnetin (30 µM), or isorhamnetin with CFTR_inh-172 (20 µM) in CD-1 mice (mean ± S.E., n = 10). * p < 0.05, ** p < 0.01.