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. 2021 Apr 12;13(4):665. doi: 10.3390/v13040665

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunological responses and virus replication in Seoul orthohantavirus (SEOV)-inoculated WKAH/hkm rats. (A) Fluctuations of IgM and IgG antibodies and IgG avidity index in rat sera. Circles, ELISA OD for IgM antibody detection; squares, ELISA OD for IgG antibody detection; triangles, IgG avidity %. Open markers are results from rat #1 and closed markers are results from rat #2. (B) Results of the ELISPOT assay. Two rats were examined by the ELISPOT assay by using control peptide #1 and epitope peptide #149. (C) Viral replication in lungs and sera was determined by a real-time PCR assay. Two rats were examined at each time point.