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. 2021 Apr 13;22(8):3983. doi: 10.3390/ijms22083983

Figure 4.

Figure 4

Mitophagy increases after UBA domain mutation. (a). JC-1was used to evaluate △φm in A2780 cells transfected with wt-p62 or ΔUBA-p62 and treated with cisplatin 5 μg/mL for 4 h. (b)., (c). After transducing lentivirus mt-keima-COX8, A2780 cells were transfected with wt-p62 or ΔUBA-p62, then treated with cisplatin 5 μg/mL for 6 h, fluorescence was visualised using microscopy and flow cytometry analysis. Scale bar, 50 μm. Data are presented as mean ± SD, n = 3. * p < 0.05 vs. wt-p62. (d). RT-PCR detection of mitochondrial copy number in A2780 cells transfected with wt-p62 or ΔUBA-p62 and treated with cisplatin 5 μg/mL for 10 h. Data are presented as mean ± SD, n = 3. * p < 0.05 vs. wt-p62. *** p < 0.001 vs. NC.