Table 2.

Summary of liquid biopsies.

Case#	Type of Fluid	CSF Source	DNA Conc (ng/mL)	Protein Conc (mg/dL)	CSF Cytology Class	Mut/WT1 VAF1(%)	Mut/WT2 VAF2(%)	Assessment
1	CSF	LP 1	228	69	I	62/19 (76.6)	50/12 (80.7)	H3K27M
2	CSF	LP 1	186	14	I	0/15 (0.0)	0/4 (0.0)	WT 2
3	CSF	LP 1	216	123	I	0/135 (0.0)	0/185 (0.0)	WT 2
4	CSF	LP 1	432	217	I	0/22 (0.0)	N/A 3	WT 2
	Plasma	N/A 3	468	N/A 3	N/A 3	2/69 (2.8)	N/A 3	H3K27M? 4
5	CSF	LP 1	264	19	I	0/7 (0.0)	1/21 (4.5)	H3K27M? 4
6	CSF	LP 1	216	32	I	1/0 (100)	2/1 (66.7)	H3K27M? 4
	CSF	LP 1	318	69	I	2/3 (40.0)	1/5 (16.7)	H3K27M? 4
7	CSF	LP 1	504	N/A 3	N/A 3	1/39 (2.5)	N/A 3	H3K27M? 4
	Plasma	N/A 5	366	N/A 3	N/A 3	1/37 (2.6)	N/A 3	H3K27M? 4
	CSF	Vent tap 5	90	N/A 3	N/A 3	2/12 (14.3)	1/9 (10.0)	H3K27M? 4
8	CSF	LP 1	216	N/A 3	N/A 3	0/0 (0.0)	0/0 (0.0)	Unreliable
9	CSF	LP 1	330	23	I	0/1 (0.0)	0/0 (0.0)	Unreliable
	CSF	Vent tap 5	306	N/A 3	N/A 3	0/0 (0.0)	0/0 (0.0)	Unreliable
10	CSF	LP 1	120	29	I	0/2 (0.0)	0/0 (0.0)	Unreliable
11	CSF	Vent tap 5	330	538	III	151/173 (46.6)	120/165 (42.0)	H3K27M
	Plasma	N/A 3	294	N/A 3	N/A 3	0/14 (0.0)	1/36 (2.8)	H3K27M? 4
12	Plasma	N/A 3	414	N/A 3	N/A 3	0/50 (0.0)	N/A 3	WT 2
	CSF	Vent tap 5	222	N/A 3	N/A 3	22/30 (42.3)	30/40 (42.9)	H3K27M

¹ Lumbar puncture, ² Wildtype, ³ Not applicable, ⁴ H3K27M suspect, ⁵ Ventricular tap.