Figure 1.

Heparan sulphate analysis in control (i.e., wild type and heterozygous combined) and MPS IIIA mice. HS accumulation in lung homogenates (A) and BALF (B) of 20-week control and MPS IIIA mice. HS was measured by LC-MS/MS using an API 4000 QTrap mass spectrometer (ABSciex) coupled with Acquity UPLC (Waters Corp). Data for A and B are expressed as disaccharide concentration (ng/mg total protein and ng/mL BALF, respectively) and plotted as mean ± SD; * indicates a significant difference between control and MPS IIIA mice. Two-tailed Students t-tests, p < 0.0001, n = 17 control and 11 MPS IIIA samples for lung tissue, n = 16 control and 13 MPS IIIA samples for BALF.