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. 2021 Apr 8;10(4):849. doi: 10.3390/cells10040849

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Protein expression of surfactant proteins -A, -B, -C and -D in BALF. (A) Representative Western blot images of part sets of SP-A, -C and -D samples pictured. Western blot analyses were performed with the following primary antibodies: SP-A (rabbit Thermo Scientific PA5-76695), SP-B (rabbit SevenHills Bioreagents WRAB-48604), SP-C (rabbit SevenHills Bioreagents WRAB-MSPC) and SP-D (rabbit Thermo Scientific PA5-51476). (B) Data normalised to total protein and presented as mean ± SD; significant differences are indicated * p = 0.0027, ** p = 0.0024 and *** p = 0.0438; two tailed Students t-test; n = 9 control, 13 MPS IIIA (SP-A analysis); 10 control, 13 MPS IIIA (SP-B analysis); 12 control, 13 MPS IIIA (SP-C analysis) and 11 control, 13 MPS IIIA (SP-D analysis) samples. Entire band height of SP-A measured from 32–45 kDa [35].