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. 2021 Apr 10;22(8):3916. doi: 10.3390/ijms22083916

Figure 2.

Figure 2

Twist regulates DDR proteins to sensitize cisplatin and PARPi in CisR OC cells. (A) Western blot analysis of Twist-knockdown with PARP1 and XRRRC1 proteins expression at day 1, 2 and 3 in OV90-CisR and SKOV3-CisR compared with the siNC group. (B,C) The treatment effect expressed as cell survival and IC50 of cisplatin and PARPi (niraparib) on CisR OV90 and SKOV3 OC cells depending on Twist expression. The both drus were treated at a various concentrations ranging from 0 to 160 µM for 72 h of incubation. CisR: cisplatin-resistant; siRNA: small interfering RNA; siNC: non-targeting negative control siRNA; siTwist: Twist siRNA. Values were represented as mean ± SD. * p < 0.05, ** p < 0.01 compared with the siNC group.