Figure 5.

Changes in [¹⁸F]1 uptake in tumor cells in response to MDM2 inhibition with SP-141 at 100 µM concentration. MCF-7, HepG2, and Hep3B cells were incubated with [¹⁸F]1 with or without SP-141 (100 µM), and the uptake was assessed after 15–120 min. Co-incubation of cells with 100 µM SP-141 resulted in a significant increase in [¹⁸F]1 uptake in wild-type p53 cell lines MCF-7 and HepG2 but not in the p53 null cell line Hep3B, potentially reflecting changes in MDM2 levels as a result of MDM2:p53 inhibition by SP-141. ** p < 0.01 and * p < 0.05 vs. the corresponding vehicle-treated cells.