Figure 3.

Alterations in Sestrins and GATOR2 after refeeding in muscle from mice in the control and alcohol-receiving groups. (A), representative immunoblots and the associated bar graph summarize data from all samples analyzed for Sestrin2 (B). For Sestrin1, Sestrin3 and Mios, there were no treatment effects (p > 0.05), and mean data are not shown. Although samples from all groups were loaded on the same gel, a gel break depicts lane(s) of the gel that have been deleted. In (C), Mios (protein component of the GATOR2 complex) was immunoprecipitated (IP) and Western blotting performed for Sestrin-1, -2, -3 and Mios. Under our assay conditions, we could not detect binding of Sestrin2 to Mios (data not shown); therefore only the association of Sestrin-1 and -3 to the GATOR2 subunit Mios was quantitated (D,E), respectively). Bar graphs represent the analyses for all immunoblots, where the mean of the fasted saline-treated control group was arbitrarily set at 100 AU. Values are mean ± SEM with n = 8/group. Only values having different superscript letters are statistically (p < 0.05) different.