Figure 8.

Dose–response curve for the knockdown efficiency of ASO (4 mice/group). LNP_ssPalmO-Phe (ssPalmO-Phe/DOPC/Chol = 40/20/40 with an additional 1.5% DMG-PEG2000) was prepared with a lipid/ASO ratio of 25 nmol/μg. Mice were injected with each of the LNP_ssPalmO-Phe–ASOap and LNP_ssPalmO-Phe–ASOgr preparations at doses in the range of 0.005–0.37 mg/kg, while ASOap and ASOgr were injected at doses in the range of 0.37–30 mg/kg. The 50% inhibitory concentration (IC₅₀) value of LNP_ssPalmO-Phe–ASOap, LNP_ssPalmO-Phe–ASOgr, ASOap and ASOgr were calculated as the dose at which they showed 50% knockdown of each target gene. LNP_ssPalmO-Phe–ASOap and ASOap: ASO targeting ApoB (a); LNP_ssPalmO-Phe–ASOgr and ASOgr: ASO targeting Nr3c1 (b). The relative mRNA expression was normalized to the saline group.