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. 2021 Apr 12;22(8):3958. doi: 10.3390/ijms22083958

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection of cell surface markers after WJ injection. Flow cytometry was employed to analyze the effect of WJ injections on pADSCs surface markers. Changes in cell size (forward light scatter; FSC-A), granularity and roughness of the cells (side light scatter, SSC-A), and the expression of mesenchymal markers CD44 and CD90 were determined. The numbers of cells expressing CD44 and CD90 are presented as a percentage (%) of the maximum (Y-axis) and their mean fluorescence intensities (MFI, X-axis). Major differences in cells markers prior to (left panels) versus after WJ injections (right panels) were not observed. Abbreviations: WJ—waterjet.