Table 4.

Statistical analyses’ results: contribution of bacterial OTUs into the principal components extracted from the matrix with relative abundance in feces of females with gallstone disease before CCE (percentage of total variance in brackets) and p-values for multiple regression with age, BMI and blood biochemistry (coefficients of determination in brackets).

OTUs: PCA 1
Main	PC 1	PC 3	PC 4		PC 6		PC 8
contributors	(40%)	(9%)	(7%)		(3%)		(2%)
Enterococcus sp.	0.862 [0.96] 3	0.00	0.00		0.00		0.00
Escherichia/Shigella sp.	0.00	0.25 [−0.65]	0.25 [−0.57]		0.07		0.01
Gemmiger	0.00	0.01	0.28 [0.67]		0.00		0.00
Faecalibacterium prausnitzii	0.01	0.63 [0.86]	0.16 [−0.38]		0.06		0.02
Akkermansia muciniphila	0.00	0.02	0.09 [0.41]		0.22 [−0.45]		0.09
Blautia luti	0.00	0.01	0.05		0.26 [−0.56]		0.02
Bifidobacterium sp.	0.00	0.03	0.02		0.07 [−0.30]		0.35 [−0.49]
Bifidobacterium sp.	0.00	0.00	0.00		0.19 [0.60]		0.27 [−0.52]
Streptococcus sp.	0.00	0.00	0.00		0.02		0.13 [0.48]
Prevotella copri	0.12 [−0.42]	0.00	0.00		0.01		0.00
OTUs: Multiple regression
R/R2	0.71/0.50	0.39/0.15		0.40/0.16		0.73/0.53		0.54/0.29
Age	0.05 4	0.16		0.34		0.55		0.11
BMI	0.03	0.10		0.92		0.09		0.35
Glucose	0.07 3	0.58		0.07		0.39		0.76
ALT	0.52	0.65		0.92		0.00		0.03
AST	0.68	0.74		0.98		0.00		0.03
Bilirubin	0.16	0.95		0.64		0.02		0.05

¹ PCA stands for principle component analysis (based on covariance). Only those principal components that (a) account for the bigger fraction of the total data variance and/or (b) displayed a statistically significant correlation with patients’ characteristics are shown. ² The values in bold show the two topmost contributions. ³ Factor loadings for variables (taxon relative abundance) are given in square brackets. ⁴ The values in bold italics and underlined italics are at p ≤ 0.05 and p ≤ 0.10, respectively.