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. 2021 Apr 9;9(4):406. doi: 10.3390/biomedicines9040406

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Qualitative evaluation of individual macrophage subpopulations from D24 and D31 (detailed donor data: Table 2). Confocal laser scanning microscopy of adherent and fluorescence-stained human hepatic macrophages was used to assess individual subpopulations. The cells from D24 with CRLM and D31 with pCCA were investigated regarding the marker (A,E) CD68 for general macrophages (yellow), (B,F) CD86 for pro-inflammatory (red), and (C,G) CD206 for anti-inflammatory macrophages (green). (D,H) The merged images show all markers simultaneously as well as the cell nuclei stained with Hoechst 33342 (blue). Images were taken using a laser scanning microscope (LSM 700, Carl Zeiss). (CRLM: colorectal liver metastasis, pCCA: perihilar cholangiocarcinoma).