Table 3.
Unconjugated primary as well as secondary antibodies for immunohistochemical stainings of human liver tissue sections. Summary of antibodies including antigen, conjugate, macrophage type, clone, isotype and host species, dilution, as well as incubation time and temperature.
| Primary Antibodies 1 | ||||||
| Antigen | Macrophage Type | Clone | Isotype, Host Species | Dilution | Incubation Time | Incubation Temperature |
| CD68 | Miscellaneous | KP1 | IgG1, mouse | 1:150 | overnight | 4 °C |
| CD80 | M1 | EPR1157(2) | IgG, rabbit | 1:1000 | overnight | 4 °C |
| CD163 | M2 | OTI2G12 | IgG1, mouse | 1:150 | overnight | 4 °C |
| Secondary Antibodies 2 | ||||||
| Antigen | Conjugate | Clone | Host Species | Dilution | Incubation Time | Incubation Temperature |
| Mouse-IgG | Peroxidase | polyclonal | rabbit | 1:200 | 2 h | RT 3 |
| Rabbit-IgG | Peroxidase | polyclonal | goat | 1:200 | 2 h | RT |
1 Purchased from Abcam (Cambridge, United Kingdom) and diluted in TBS supplemented with 1% BSA and 0.03% Triton X-100, 2 Purchased from Sigma and diluted in TBST, 3 RT: room temperature.