Figure 3.

Evaluation of supernatant change from Cre⁺ to reporter cells. HUVEC (A), iPSC-ECFC (B), and ASC (C) reporter cells were cultured under different conditions. Reporter cells cultured in full medium were used as negative control (untreated). Furthermore, as positive control reporter cells were transfected with the Cre construct. Supernatant of HUVEC, hiPSC-ECFC, or ASC Cre⁺ cells were used for culturing reporter cells. Quadrant regions show the percentage of cells in each subpopulation either expressing dsRed (PE, Q1), eGFP (FITC, Q3), both (Q2), or non-fluorescent (Q4). Single green cells are indicated by the white arrows. Data presented was obtained from three independent experiments using two different donors for HUVEC and ASC and one cell donor for hiPSC-ECFC. Scale bar: 100 µm.