Table 4.
Mean values of measured in vitro fermentation parameters in a 24 h incubation period of species harvested in 2018 and 2019 consecutively. Numbers between brackets correspond to the standard error of the mean (SEM).
| Species | Partner Prop. |
Total Gas (mL/200 mg DOM 1) | Methane (mL/200 mg DOM) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2018 | 2019 | 2018 | 2019 | ||||||||||
| L. perenne | 1 | 93.4 | (3.23) | 80.8 | (2.38) | 19.2 | (1.68) | 15.6 | (0.24) | ||||
| C. carvi | 0.25 | 94.2 | (0.68) | 83.9 | (0.68) | 16.1 | (0.07) | 16.4 | (0.32) | ||||
| 0.375 | 89.5 | (1.94) | 82.4 | (0.64) | 16.5 | (0.45) | 16.2 | (0.33) | |||||
| 0.5 | 90.0 | (0.72) | 74.9 | (0.41) | 15.3 | (0.34) | 14.5 | (0.16) | |||||
| 0.675 | 86.8 | (1.14) | 82.2 | (0.72) | 14.3 | (0.09) | 16.1 | (0.08) | |||||
| 0.75 | 87.7 | (1.22) | 77.8 | (0.64) | 16.0 | (0.43) | 15.4 | (0.45) | |||||
| 1 | 83.8 | (0.95) | 77.3 | (5.31) | 16.6 | (0.46) | 15.1 | (1.29) | |||||
| C. intybus | 0.25 | 94.3 | (0.28) | 81.2 | A | (1.10) | 16.0 | (0.06) | 15.9 | AB | (0.28) | ||
| 0.375 | 86.2 | (1.40) | 81.3 | A | (0.55) | 14.4 | (0.17) | 16.2 | A | (0.13) | |||
| 0.5 | 88.1 | (1.57) | 75.3 | AB | (0.74) | 15.4 | (0.22) | 14.4 | AB | (0.07) | |||
| 0.675 | 86.2 | (1.66) | 80.9 | A | (0.69) | 13.6 | (0.22) | 15.9 | AB | (0.09) | |||
| 0.75 | 84.8 | (1.10) | 75.5 | B | (1.49) | 15.3 | (0.38) | 14.7 | B | (0.55) | |||
| 1 | 79.5 | (0.69) | 72.7 | AB | (0.25) | 15.7 | (0.62) | 14.0 | AB | (0.15) | |||
| L. corniculatus Leo | 0.25 | 91.4 | AB | (2.25) | 78.8 | AB | (1.16) | 16.7 | AB | (0.24) | 15.3 | A | (0.19) |
| 0.375 | 88.0 | AC | (1.34) | 79.6 | A | (0.50) | 16.9 | A | (0.41) | 16.3 | AB | (0.08) | |
| 0.5 | 83.6 | CD | (1.28) | 76.7 | AB | (1.00) | 16.2 | AB | (0.18) | 14.9 | A | (0.18) | |
| 0.675 | 81.9 | ABCD | (2.03) | 75.7 | ABC | (1.46) | 14.7 | AB | (0.17) | 15.8 | AB | (0.22) | |
| 0.75 | 83.6 | jBD | (1.05) | 70.9 | kBC | (0.73) | 16.2 | B | (0.44) | 14.8 | AB | (0.72) | |
| 1 | 75.7 | BD | (0.69) | 63.8 | C | (2.23) | 16.5 | AB | (0.37) | 12.8 | B | (0.23) | |
| T. pratense | 0.25 | 87.1 | A | (1.84) | 86.5 | (0.87) | 16.0 | A | (0.34) | 17.1 | A | (0.28) | |
| 0.375 | 82.8 | ABC | (2.33) | 87.6 | (0.40) | 16.5 | A | (0.53) | 17.5 | AB | (0.37) | ||
| 0.5 | 82.9 | AB | (0.72) | 86.3 | (1.07) | 16.6 | AB | (0.27) | 17.5 | AB | (0.34) | ||
| 0.675 | 80.2 | ABC | (1.82) | 89.4 | (2.02) | 14.9 | AB | (0.15) | 18.3 | B | (0.35) | ||
| 0.75 | 80.1 | BC | (3.20) | 87.2 | (2.06) | 16.8 | B | (0.35) | 18.5 | B | (0.08) | ||
| 1 | 72.0 | C | (0.78) | 82.8 | (1.06) | 16.7 | AB | (0.62) | 17.3 | B | (0.20) | ||
| T. repens | 0.25 | 96.0 | A | (0.80) | 81.4 | AB | (0.67) | 17.1 | (0.03) | 16.4 | (0.37) | ||
| 0.375 | 82.9 | AB | (1.62) | 80.1 | AB | (0.23) | 16.5 | (0.44) | 16.4 | (0.41) | |||
| 0.5 | 89.1 | ABC | (0.55) | 75.6 | AC | (0.44) | 16.7 | (0.15) | 15.0 | (0.43) | |||
| 0.675 | 81.5 | ABC | (0.69) | 79.3 | AB | (1.04) | 15.3 | (0.08) | 16.6 | (0.22) | |||
| 0.75 | 77.9 | C | (1.16) | 73.5 | CD | (1.19) | 16.3 | (0.51) | 16.3 | (0.41) | |||
| 1 | 75.4 | BC | (0.90) | 69.8 | BD | (0.20) | 17.5 | (0.55) | 15.1 | (0.04) | |||
| P. lanceolata | 0.25 | 95.3 | A | (0.95) | 82.4 | (1.83) | 16.4 | ABCD | (0.11) | 16.2 | (0.57) | ||
| 0.375 | 87.0 | AB | (0.84) | 79.6 | (0.82) | 15.2 | AB | (0.10) | 15.8 | (0.20) | |||
| 0.5 | 87.5 | A | (0.82) | 82.6 | (0.53) | 16.5 | AC | (0.23) | 16.4 | (0.09) | |||
| 0.675 | 83.9 | AB | (1.54) | 79.5 | (1.14) | 14.3 | CD | (0.24) | 15.9 | (0.36) | |||
| 0.75 | 81.2 | AB | (1.40) | 77.5 | (2.36) | 15.4 | ABCD | (0.43) | 15.7 | (0.45) | |||
| 1 | 77.3 | B | (1.50) | 72.6 | (4.55) | 16.1 | BD | (0.37) | 13.7 | (0.75) | |||
1 DOM: digestible OM; j,k noncommon lowercase letters indicate differences between years; A–D noncommon uppercase letters indicate differences between partner proportions.