Figure 5.

Single-cell RNA sequencing application in drug-response prediction. Targeted cell populations are selected from human organs/tissues and then one type of cell population can be sorted. Drug-dependent gene mutations and drug specificity of targeted genes can be defined after selected cells are treated with drugs (B, brown arrows). On the other hand, gene sequences and epigenetics of selected cells can be measured before the cell sorting (C, black arrows) to compare the cell population with single-cell sequences. Molecular mechanisms, drug efficacy, and toxicity of identified core/driver genes and networks can be validated by editing target genes. Reprinted with permission from ref. [157]. Copyright 2017 SpringerLink.