Figure 1.

Cell viability of ARPE-19 cells treated with quercetin only or quercetin plus NaIO₃. (A) ARPE-19 cells were treated with various concentrations of quercetin (1.25, 2.5 and 5 μM) for 24 h, and the cell viability was measured using a CCK-8 assay. (B) ARPE-19 cells were pretreated with various concentrations of quercetin (1.25, 2.5 and 5 μM) for 1.5 h and then with NaIO₃ (6 mM) for 24 h, before cell viability was measured using the CCK-8 assay. (C) ARPE-19 cells were pretreated with various concentrations (1.25, 2.5 and 5 μM) of quercetin for 1.5 h before treatment with NaIO₃ (6 mM) for 15 h. ARPE-19 cells were then detected by flow cytometry after staining with both Annexin V-FITC and PI for 30 min. (D) The percentage of apoptotic cells in each treatment group was quantified. Values represent the mean ± SD (n = 3). Data bars without letters in common (a–d) indicate a significant difference (p < 0.05).