Figure 3. Biofilm production is significantly reduced in the presence of silver acetate.

A. baumannii cultures were incubated statically in Mueller-Hinton broth at 37 °C in 24-well plates in the presence of varying concentrations of silver acetate (0–7.81 mg L⁻¹). (A) After incubation the wells were washed and stained with crystal violet to determine the amount of biofilm formed (OD₅₄₀). Differences in biofilm formed between the lower (0–1.95 mg L⁻¹) and higher (3.91–7.81 mg L⁻¹) concentrations of silver acetate were highly significant (ANOVA and Tukeys multiple comparison test, ****P < 0.0001). (B) After incubation, biofilms were disaggregated and bacterial viability was determined. Significant differences in biofilm formation were observed between the lowest (0–0.488 mg L⁻¹) and highest concentrations of silver acetate (3.9–7.8 mg L⁻¹, ANOVA and Tukey’s multiple comparison, **P = 0.0005–0.003) N = 3 ± SEM.