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Expression of CD63-BirA constructs alone or in the presence of LMP1 exhibit biotinylase activity. (A) Protein expression and BirA activity were verified using indicated antibodies. (B) Biotinylated proteins were pulled-down from the total cell lysate using streptavidin magnetic beads. Pull-down efficiency was verified by Western blotting using the indicated antibodies. WCL: whole cell lysate; PD, pull-down.
