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. 2021 Apr 15;22(8):4100. doi: 10.3390/ijms22084100

Figure 3.

Figure 3

Mitochondria appear more fragmented in doxycycline-treated H9C2 cells. (A–C), representative H9C2 cells loaded with TMRM with corresponding masks for quantification. (D–F), Mitochondria in doxycycline-treated H9C2 cells appeared more fragmented, were smaller, had lower aspect ratios and higher circularity compared to vehicle. Values are median (area and aspect ratio) or 25% percentile values (circularity) because of non-normal distribution. n = 5–12 independent samples per group on four different days. Error bars are SEM. Scale bar: 10 μm. *: p < 0.05 vs. vehicle; #: p < 0.05 vs. 10 µg/mL doxycycline, one-way ANOVA followed by Tukey’s post-hoc test for multiple comparisons.