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. 2021 Apr 15;26(8):2293. doi: 10.3390/molecules26082293

Figure 7.

Figure 7

Figure 7

Florescent images of NF-κB inhibition. A549 cells were treated with TNFα (2.5 ng/mL) and/or different positive QS CP derivatives then fixed and immunostained with anti-p65 and anti-mouse IgG conjugate to Alexa AF488 (green) and nuclear staining with DAPI (blue). (A) The negative controls were: untreated, treated with the solvent DMSO (0.16%) for 120 min, TNFα (2.5 ng/mL) for 15 min, DMSO (0.16 %) for 120 min with TNFα (2.5 ng/mL) for 15 min. (B) CP-154 and CP-158 at 160 μM for 120 min with and without TNFα (2.5 ng/mL) for 15 min. (C) CP-286 and CP-215 at 160 μM for 120 min with and without TNFα (2.5 ng/mL) for 15 min. (D). Curcumin (CU) was used as a positive control with the same experimental design as in (B) or (C).