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. 2021 Apr 15;22(8):4102. doi: 10.3390/ijms22084102

Figure 3.

Figure 3

RRM1 gene and protein expression in RRM1 highly expressed human bladder cancer cells after transfection with adenoviral vectors. RRM1 gene expressions were effectively inhibited after Ad-shRRM1 infection at MOI = 10 in (A) 253J cells and (B) TR112 cells, time-dependently. RRM1 protein expression can be seen in RRM1 highly expressed (C) 253J and (D) RT112 cells. Quantification of the PBS, Ad-shScramble and Ad-shRRM1 ration of RRM1/GAPDH protein expression (E). Western blot analyses were performed at 72 h after transduction with adenoviral vectors at MOI = 20 and one of three experiments with similar results is shown. (MOI: multiplicity of infection; * p < 0.05, ** p < 0.005 vs. Ad-shScramble treatment).