Figure 2.

Top-ranked urinary metabolites that differentiate CD from UC pediatric patients (p < 0.05) following sum (or osmolality) normalization when using a Mann–Whitney U-test. (A) Box-whisker plots for 10 urinary metabolites, including unknown metabolites annotated based on their characteristic accurate mass, relative migration time and ion detection mode (m/z: RMT:mode) were subsequently identified by collision induced dissociation with MS/MS, as shown for (B) 5-hydroxy indoxyl sulfate. (C) Receiver operating characteristic (ROC) curves are depicted for two top-ranked ratiometric urinary biomarkers following glog transformation with good discrimination potential for the two major subtypes of pediatric IBD in affected children (AUC > 0.960–0.970, p < 3.2 × 10⁻⁵).